Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Cavicide (Warehouse, Cat # 79300360) 6. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Note: In this user guide, SpectraMax ® refers to several SpectraMax . No accessories are needed and SoftMax® Pro. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. 1. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. Open the SoftwarePro® Data Acquisition & Analysis software,. The chamber contains springs that position the cuvette in the proper alignment for a read. 2. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. These robust readers have been placed in labs from. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Step 3. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Fluorescence Microplate Reader Comparison Chart. Download PDF versions of the SpectraMax M5 User Guides from this page. Pick the best speed, sensitivity, and price for your needs. Step 4: Incubate at room temperature for 1 hour, protected from light. Figure 4: Transcreener ADP. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. , contact Molecular. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Monday to Friday 7am - 5pm Pacific Time. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. It may be installed in the top or bottom read detection cartridge transport. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. spr SpectraMax Plus 384. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. Lift up the lid over the chamber to insert or remove a cuvette. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 3. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. The SpectraMax M5 is a microplate reader that can be used for various applications,. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. $4,499. Exhibit with us {{>currencyLabel}} Back. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 4. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. Step 2. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SoftMax® Touch Software is installed in. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. Italic and boldface type are used for emphasis. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. . すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. . Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 1) EarlyTox Glutathione Assay (6. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. Other technologies such as imaging. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). edu) to be added as a trained user in iLab. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. Setup recommendations for SpectraMax® M5e. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Figure 2. . KeySpan adapter for SoftMax Pro and SpectraMax readers. The M5 can read volumes down to 2uL with the. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Three kits covering a wide range of dsDNA concentrations. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. 3. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. . free shipping. . In this product video, see how the SpectraMax M5 quickly reads samples. 4. Description 1. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 3. Dual monochromators provide the ability for. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Cavicide (Warehouse, Cat # 79300360) 6. Wavelength optimization. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 6. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. SpectraMax DuoLuc Reporter Assay (SMP 6. Cables. Spectral Range. 190 - 1100 nm. 00 USD. While they play an important role in generating data from a wide range of. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Add to Cart. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. The chamber. The drawing shows a standard microplate of 15 mm height covered with a lid. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. 0 out of 5 $ 5,999. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. The M5 can read volumes down to 2uL with the uMax Low. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Select a rugged, lightweight model designed to be used in the field. 9000-0756. . Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. $2,264. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. Multimode Reader Comparison Chart. 000). +44-118-944-8000. Note: In this user guide, all references to SpectraMax Multi-Mode. 1. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. . The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. . Example: [Drawer]. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 4. , ~488 nm), has the optical. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. All reagents were brought to room temperature before use. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. 3: The cuvette chamber. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Also refer to this Multimode Reader Comparison Chart. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. UV/Vis Photometric Performance:. 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. For example, microplates containing different samples can be read using the312 gain calibration failed. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Click SoftMax Pro Software User Guide. 3: The cuvette chamber. . Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . For very concentrated samples, use a minimum2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. . Description 1. 9000-0756. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. The following document is intended to demonstrate setup of this. Table of Contents. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Five preferred modes for diverse bioresearch applications. Plate chamber design of the SpectraMax category 1 microplate readers. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Read fluorescence polarization at room temperature (RT, 19–25°C). . View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. 7" color, capacitive touch screen. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. 1 or greater versions. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. Cytokines in medium released. Description. The. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 315 can't find zero order. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. Bookings are free, but you will need to use iLab to book. The drawing shows a standard microplate of 15 mm height covered with a lid. Step 1: Open the SoftMax Pro IMAP FP protocol. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. . It is available to all researchers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 305 reference level saturation. . Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . Published Dec 16, 2020 | Updated Dec 16, 2020. 4. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Methods Preparation of reagents and standards. I have been using the SpectraMax M5 since 2011. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Instructions on the proper use of the SpectraMax M5 Plate Reader. e. . Principle of the HTRF IP-One competitive binding assay. . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. No filter change with dual monochromator optics. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 0 out of 5 $ 15,999. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 00 USD. 313 reference gain check fail. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). Category 2 and 3 microplate readers use gradient heating as well. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Detection of pro-inflammatory factors. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. The dual monochromator optics allow the widest range of applications to be utilized for bior. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. These all-inclusive assay kits are ready to use in assay validation or screening. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Sale. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Add to Cart. Softmax Pro GxP Software 6. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Offer Ends In: 18 Days 19: 38: 21. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Table of Contents. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax M5 microplate reader. 72 MB. 5. Set the excitation at 531 nm and emission at 595 nm. The SpectraMax M5 Multi-Mode. type Clear. 40266 Email: drugdiscoverytech@lifetech. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Add to Cart. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. Find regional support. These lab workhorses are used in drug discovery, research, bioassay validation, and. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The chamber contains. 2. 1. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. Bookings are free, but you will need to use iLab to book. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). com A. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Examples: “Press carefully to engage,” “ Do not. Step 1: Open the SoftMax Pro IMAP FP protocol. Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Sale. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 1) EarlyTox NucView 488 Assay (6. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. free tracer decreases, resulting in an. The KeySpan USA-19HS is often required with new Windows 7. 1. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. Offer Ends In: 18 Days 19: 38: 21. I have been using the SpectraMax M5 since 2011. Molecular Devices. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The plate reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. e. . 1. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. 3. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. the IMAP Platform and smoothly transition into high-throughput screening. With optimized reagents, option to operate the readers. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. 5. It does not work as expected. 40266 Email: drugdiscoverytech@lifetech. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The following document is intended to demonstrate setup of this instrument. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). SpectraMax Injector Cartridge with SmartInject™ Technology. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . 00 USD. 0 mm wide, and heights from 14 mm to 15 mm. , ~488 nm), has the optical. 1. 2. The following document is intended to demonstrate setup of this instrument. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World.